Leishman’s stain is used in microscopy for staining blood smears. It is generally used to differentiate and identify leucocytes, malaria parasites, and trypanosomas. It is based on a mixture of methylene blue and eosin.

Biological Dye
Stain for Blood Smears
Romanowsky Stain

Molecular Formula: C24H16N2O6
Molecular Weight: 428.39 g/mol
Maximum Absorption Lambda 1 in Methanol: 640-645nm
Maximum Absorption Lambda 2 in Methanol: 522-525nm
Absorptivity(A1%/1cm in Methanol): >900
Absorptivity(A1%/1cm in Methanol): >650
Loss on Drying: <5%

Hazard Phrases: Not considered harmful under normal laboratory conditions

Cas No: 12627-53-1
Einecs No: 235-732-1